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cycloheximide  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cycloheximide
    Cycloheximide, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 411 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cycloheximide/product/Cell Signaling Technology Inc
    Average 96 stars, based on 411 article reviews
    cycloheximide - by Bioz Stars, 2026-05
    96/100 stars

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    Effects of functional uORFs in the 5′-UTR of the eg1 gene on gene expression in P . oxalicum grown on Avicel. (a) RT-qPCR analysis of gfp transcription. (b) RT-qPCR assay of eg1 transcription in OE- eg1 - muORF mutants. (c) mRNA stability of eg1 following 6 h dactinomycin pretreatment. (d) mRNA stability of eg1 following 2 h <t>cycloheximide</t> pretreatment. Data values represent mean ± standard deviation. Each experiment was performed with at least three biological replicates. ∗ p < 0.05 and ∗∗ p < 0.01 denote statistically significant differences compared to the control strain eg1 -5′-UTR- GFP or OE eg1 .
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    Effects of functional uORFs in the 5′-UTR of the eg1 gene on gene expression in P . oxalicum grown on Avicel. (a) RT-qPCR analysis of gfp transcription. (b) RT-qPCR assay of eg1 transcription in OE- eg1 - muORF mutants. (c) mRNA stability of eg1 following 6 h dactinomycin pretreatment. (d) mRNA stability of eg1 following 2 h <t>cycloheximide</t> pretreatment. Data values represent mean ± standard deviation. Each experiment was performed with at least three biological replicates. ∗ p < 0.05 and ∗∗ p < 0.01 denote statistically significant differences compared to the control strain eg1 -5′-UTR- GFP or OE eg1 .
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    Effects of functional uORFs in the 5′-UTR of the eg1 gene on gene expression in P . oxalicum grown on Avicel. (a) RT-qPCR analysis of gfp transcription. (b) RT-qPCR assay of eg1 transcription in OE- eg1 - muORF mutants. (c) mRNA stability of eg1 following 6 h dactinomycin pretreatment. (d) mRNA stability of eg1 following 2 h cycloheximide pretreatment. Data values represent mean ± standard deviation. Each experiment was performed with at least three biological replicates. ∗ p < 0.05 and ∗∗ p < 0.01 denote statistically significant differences compared to the control strain eg1 -5′-UTR- GFP or OE eg1 .

    Journal: Synthetic and Systems Biotechnology

    Article Title: Modifying the upstream open reading frames of cellulase gene enhances cellulase production in Penicillium oxalicum

    doi: 10.1016/j.synbio.2025.12.016

    Figure Lengend Snippet: Effects of functional uORFs in the 5′-UTR of the eg1 gene on gene expression in P . oxalicum grown on Avicel. (a) RT-qPCR analysis of gfp transcription. (b) RT-qPCR assay of eg1 transcription in OE- eg1 - muORF mutants. (c) mRNA stability of eg1 following 6 h dactinomycin pretreatment. (d) mRNA stability of eg1 following 2 h cycloheximide pretreatment. Data values represent mean ± standard deviation. Each experiment was performed with at least three biological replicates. ∗ p < 0.05 and ∗∗ p < 0.01 denote statistically significant differences compared to the control strain eg1 -5′-UTR- GFP or OE eg1 .

    Article Snippet: The medium was supplemented with either dactinomycin (Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China) at a final concentration of 5 μg/mL or cycloheximide (MedChemExpress, Monmouth Junction, NJ, USA) at a final concentration of 10 μg/mL.

    Techniques: Functional Assay, Gene Expression, Quantitative RT-PCR, Standard Deviation, Control